The isolation of mumps virus from the
blood of a patient has been reported on but
one occasion, in 1949.1 The lack of subsequent
confirmatory reports, then, leaves the
question of viremia in mumps somewhat
in doubt. The present paper reports three
subsequent isolates from the sera of two
Materials and Methods
Serum and spinal fluid to be
tested for the presence of virus were injected
undiluted into the amniotic sac of 7-day-old
embryonated eggs. Each egg received 0.2 ml. of
inoculum. Eggs were incubated for six days at
37 C and then chilled overnight at 4 C. The eggs
were then opened and the allantoic and amniotic
fluids removed separately. The fluids from each
egg were then tested for the presence of a hemagglutinin
by addition of 0.5% chicken red blood cells.
When no virus was obtained on primary passage,
two blind passages were made with pooled
amniotic fluids. If negative
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