Radioimmunoassay of Parathyroid Hormone

G. D. Aurbach, MD; John T. Potts Jr., MD
Arch Intern Med. 1969;124(4):413-416. doi:10.1001/archinte.1969.00300200025004.
Text Size: A A A
Published online

The technic of radioimmunoassay developed by Berson and Yalow 1 is based on the competitive inhibition of binding of radioactively labeled hormone to specific antibody. There must be available pure hormone antigen for labeling with radioisotope, usually iodine 131 or iodine 125. The ratio of antibody-bound hormone labeled with 131I (B) to free hormone labeled with 131I (F) is a function of hormone concentration. By measuring the ratio B/F after incubation with dilute mixtures of antibody and labeled hormone, a sensitive immunoassay can be obtained which can be applied to detection of hormone in peripheral plasma. A standard curve for the assay of parathyroid hormone is illustrated in Fig 1. The graphical analysis shown provides more than quantitation alone; by analyzing the shape of the curves one can derive important qualitative information regarding the nature of an unknown hormone sample. For example, a hormonal preparation immunological identical to


Sign In to Access Full Content

Don't have Access?

Register and get free email Table of Contents alerts, saved searches, PowerPoint downloads, CME quizzes, and more

Subscribe for full-text access to content from 1998 forward and a host of useful features

Activate your current subscription (AMA members and current subscribers)

Purchase Online Access to this article for 24 hours

First Page Preview

View Large
First page PDF preview





Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).
Submit a Comment


Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

Related Content

Customize your page view by dragging & repositioning the boxes below.