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The Chromatography of Steroids.

Harold P. Schedl, MD, PhD
Arch Intern Med. 1965;116(3):469. doi:10.1001/archinte.1965.03870030149035.
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This is a comprehensive, well-balanced treatise on both the theoretical and practical aspects of chromatography. The basic theory and general principles of chromatography are succinctly presented and then applied in a very practical way to the steroids. The primary emphasis is on paper chromatography, in which the author has made many noteworthy contributions. Much new, useful, and practical information is included in the section on techniques and apparatus. Quantitative chromatography, both colorimetric and radioisotopic, are very well treated with primary emphasis on paper chromatography. The use of chromatographic methods for structural analysis and identification of steroids is developed as a valuable application of chromatography. The section describing typical analytical problems in steroid biochemistry covers all the major hormones. There is a glossary of terms and nomenclature and appendices describing purification of reagents and adsorbants, microchemical reactions for steroids, and methods of detections of steroids on paper chromatograms.

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