September 1951, Vol 88, No. 3 >

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ARTICLE | September 1951

THE DIVIDED CULTURE PLATE: Its Use in Testing for Sensitivity to Antibiotics

JOSEPH FELSEN, M.D.; ALFRED J. WEIL, M.D.

AMA Arch Intern Med. 1951;88(3):406-408. doi:10.1001/archinte.1951.03810090137015.

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The divided culture plate¹ was devised principally as a practical aid in the bacteriologic diagnosis of enteric infection and for antibiotic studies. It consists of the standard 94-mm. Petri-dish bottom, which is separated into quarters by two smooth bisecting ridges of glass 5.5 mm. in height. The inside height of the bottom is 13 to 15 mm., and the covers are identical with those of ordinary Petri dishes. The quadrants are marked I to IV for easy identification. In studies on enteric pathogens, the suspected material (feces or, preferably, exudate obtained by mucosal crypt aspiration²) is seeded on the quadrants, each of which contains a different culture medium chosen for its selectivity (e. g., Endo's [fuchsin sulfite agar], S. S. [Salmonella-Shigella thiosulfate-citrate bile agar], desoxycholate citrate agar, and MacConkey's [bile-salt agar] or E. M. B. [eosin methylthionine chloride agar]). The plates are incubated overnight and suspicious colonies picked

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FELSEN J, WEIL AJ. THE DIVIDED CULTURE PLATE: Its Use in Testing for Sensitivity to Antibiotics. AMA Arch Intern Med. 1951;88(3):406-408. doi:10.1001/archinte.1951.03810090137015.

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