During the course of some observations on dogs after experimental obstructive jaundice had been produced, we were surprised at the frequency with which a reduction of the plasma chlorides occurred after complete obstruction of the duct and cholecystectomy in the same animal. The degree of reduction in the sodium chloride varied somewhat in different animals, but in all animals in which the obstruction was complete and in which the gallbladder had been removed, some depression in the amount of chlorides resulted. This behavior of the plasma chlorides may be, and probably is, only one part of an alteration in the entire physicochemical system of the animal, but it is most striking. The results here published are merely part of a more extensive study now being carried out.
In 1923, in a paper on "Observations on the Regulation of Osmotic Pressure," Gram1 published some results of estimations on the plasma chlorides