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THE NATURE OF SPECIFIC HEMOLYSINS AND STANDARD METHOD OF PREPARING ANTISHEEP HEMOLYSIN

L. G. HADJOPOULOS, M.D.
Arch Intern Med (Chic). 1921;27(4):441-448. doi:10.1001/archinte.1921.00100100057005.
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From the time of the discovery of specific antibodies and their subsequent application to the so-called complement fixation tests, the study of specific hemolysins has especially attracted the attention of numerous investigators.

As early as 1875, Landois,1 in the course of transfusion experiments, noticed the toxic (hemolytic) effect of alien serums on the erythrocytes of the recipients. But our knowledge of specific hemolysins dates back to 1898, when Belfanti and Carbone,2 and almost simultaneously Bordet,3 published their observations on the gradually acquired hemolytic properties of serums of animals injected with erythrocytes of other species.

Following the above observations, more important facts about the multiplicity of such lytic antibodies were disclosed, and so, the general interest was more concentrated on bacteriolysins and other kinds of cytolysins that have to do more directly with the all important question of immunity and anaphylaxis. This resulted in a partial neglect of the equally important

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