All samples from the same patient were run in duplicate in the same assay. Levels of CRP were measured by enzyme-linked immunosorbent assay (intra-assay coefficient of variation [CV] of 1.7%-3.9%; sensitivity, 0.002 mg/L; Diagnostic Systems Laboratories Inc, Webster, Tex). Adiponectin was measured by radioimmunoassay (CV, 1.8%-3.6%; sensitivity, 0.001 μg/mL; LINCO Research Inc, St Charles, Mo). Sandwich enzyme immunoassay technique was performed for TNF-α (CV, 5.3%-8.8%; sensitivity, 0.12 pg/mL), sTNFR1 (CV, 3.6%-5.0%; sensitivity, 0.001 ng/mL), sTNFR2 (CV, 2.6%-4.8%; sensitivity, 0.001 ng/mL), and IL-6 (CV, 6.9%-7.8%; sensitivity, 0.039 ng/L) using kits from R&D Systems Inc, Minneapolis, Minn. Insulin was measured by radioimmunoassay (CV, 4.9%-10%; Diagnostic Products Corp, Los Angeles, Calif). The concentration of fibrinogen (CV, <1.50%) was determined using an immunoturbidimetric assay with reagents and calibrators from Kamiya Biomedical Co (Seattle, Wash). Nonesterified fatty acid concentrations were measured using an in vitro enzymatic colorimetric assay kit (CV, 1.1%-2.7%; Wako Chemicals USA Inc, Richmond, Va). Complete blood cell count and glucose and lipid concentrations were measured by standard technique.